We are all familiar with genetics or gene, which is made up of DNA. Many young people will have first learned about genetics and DNA from the legendary film Jurassic Park, which talked about DNA and genetics to create dinosaurs. However, humans also have DNA, and for us, it can influence everything around us. Everyone... Continue reading
According to Peter Crosta (UoI), "viruses are microscopic organisms that exist almost everywhere on the planet. They can infect animals, plants, fungi and even bacteria..... Viruses vary in complexity. They consist of genetic material, RNA or DNA, surrounded by a protein, lipid (fat) or glycoprotein envelope". Talking about viruses can be complicated because each one... Continue reading
ELISA (Enzyme-linked Immunosorbent Assay) is a plate-based assay technique designed to detect and quantify soluble substances such as peptides, proteins, antibodies and hormones. Other names, such as enzyme immunoassay (EIA), are also used to describe the same technology. In an ELISA, the antigen (target macromolecule) is immobilized on a solid surface (microplate) and then forms... Continue reading
Immunoglobulin anti-D (lgD) secondary antibodies or Immunoglobulin D (lgD) is a monomeric antibody isotype that is expressed on the plasma membranes of immature B-lymphocytes. Anti-D immunoglobulin (lgD) is also produced in a secreted form found in small amounts in blood serum. The secreted IgD consists of two heavy chains of the delta class and two... Continue reading
Flow cytometry is a biophysical technology based on the use of laser light. In other words, flow cytometry is an analytical method that allows the rapid measurement of certain physical and chemical characteristics of cells or particles suspended in liquid that produce a signal individually when interfered with by a light source. This light is... Continue reading
Recombinant DNA technology is playing a key role in improving health conditions through the development of new vaccines and pharmaceuticals. Treatment strategies are also improved through the development of diagnostic kits, monitoring devices and new therapeutic approaches. The synthesis of synthetic human insulin and erythropoietin using genetically modified bacteria and the production of new types... Continue reading
"Recombinant Rabbit Polyclonal Antibodies are composed of a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. Recombinant rabbit polyclonal antibodies provide the best of both worlds: the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies, all with the consistency found only in a recombinant antibody. Although they are... Continue reading
This article will discuss the PCR protocol in depth to provide clarity on this topic. Once you have your genetic samples, one of the first steps in PCR is to design the primers needed to perform the PCR reaction. The first step in proceeding with a standard PCR is to design the primers. You will... Continue reading
The history of cloning and its characteristics have evolved over time, advancing and giving rise to modern cloning, in this article we will review its evolution and how it is currently applied. Cloning is a technique used by scientists to make exact genetic copies of living beings. Genes, cells, tissues and even whole animals can... Continue reading
The Branched DNA Assay (bDNA) provides a unique and powerful tool for the reliable quantification of nucleic acid molecules. Fundamentally different from target amplification methods such as PCR, the bDNA assay directly measures nucleic acid molecules at physiological levels by amplifying the reporter signal, rather than replicating the target sequences as a means of detection,... Continue reading
