Recombinant Rabbit Polyclonal Antibodies 19th November 2021 – Tags: , , ,

What are rabbit polyclonal antibodies?

“Recombinant Rabbit Polyclonal Antibodies are composed of a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. Recombinant rabbit polyclonal antibodies provide the best of both worlds: the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies, all with the consistency found only in a recombinant antibody.

Although they are functionally the same as polyclonal antibodies, recognising multiple epitope sites on the target and yielding higher detection sensitivity for low abundance targets, the exact population of heavy and light chains in a recombinant polyclonal antibody can be produced in each batch, avoiding the biological variability typically associated with polyclonal antibody production.

Polyclonal antibodies (pAbs) are antibodies secreted by different B cell lineages in the body (whereas monoclonal antibodies come from a single cell lineage).

They are a collection of immunoglobulin molecules that react against a specific antigen, each of which identifies a different epitope. These antibodies are usually produced by inoculation of a suitable mammal, such as chickens, goats or rabbits. However, the rabbit is the most commonly used laboratory animal for this purpose.

recombinant rabbit polyclonal antibodies production

Recombinant rabbit polyclonal antibody production

Recombinant rabbit polyclonal antibodies or  “Recombinant Rabbit Polyclonal Antibodies” have a similar consistency to other polyclonal antibodies, but offer a non-animal option. Rabbits are immunised and, instead of using hybridomas, peripheral blood mononuclear cells (PBMC) are isolated and screened.

After library cloning and screening, their heavy and light chain cDNAs are co-expressed using a mammalian-optimised expression cassette. The resulting DNA is used for the production of non-animal origin formulations by cell culture.

Advantages of Recombinant Rabbit Polyclonal Antibodies

Recombinant rabbit polyclonal antibodies exhibit superior batch-to-batch reproducibility because the stored DNA does not exhibit cellular drift, a phenomenon in which hybridomas change their expression patterns over time. Because the selection processes used in the manufacture of recombinant rabbit polyclonal antibodies involve three different selection steps, the resulting antibody has better specificity and sensitivity than standard antibodies.

  • Other advantages are:
    Improved specificity and sensitivity compared to other polyclonal antibodies
  • Batch-to-batch consistency thanks to recombinant technology
  • Formulations without animal origin, resulting from cell culture production
  • Improved antibody performance due to multiple screening tests during development
Mouse IFN-beta ELISA

Features Mouse IFN-beta ELISA

Optimised capture and detection antibody pairings with recommended concentrations save significant development time
Development protocols are provided to guide assay optimisation
Assay can be tailored to your specific needs
Cost-effective alternative to complete kits

SOCS-1 Antibody

SOCS1 is a member of the STAT inhibitor (SSI) family, also known as suppressors of cytokine signalling (SOCS). Members of the SSI family are negative regulators of cytokine signalling. SOCS1 functions downstream of cytokine receptors and participates in a negative feedback loop to attenuate cytokine signalling.

Polyclonal antibodies are produced by immunising animals with a synthetic peptide corresponding to the residues surrounding Ala156 of human SOCS1. The antibodies were purified by protein A and peptide affinity chromatography.

Anti-SLC1A5 Antibody

Several suppliers offer anti-SLC1A5 antibodies. This gene encodes the solute carrier family 1 member 5 protein in humans and may also be known as ASCT2, AAAT, ATBO, M7V1, neutral amino acid transporter B(0), and ATB(0). Structurally, the protein has a mass of 56.6 kilodaltons. Based on the gene name, canine, porcine, monkey, mouse and rat orthologues can also be found.

References

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