mononuclear cells Continue reading...

Mononuclear cells 18th June 2022

Mononuclear cells, or Total Nuclear Cells, are mesenchymal stem cells involved in tissue regeneration and repair processes. They are primitive, non-specialised cells that have the ability to transform themselves into different types of cells in the body. Their function is to replace tissue damaged as a result of trauma or ageing. What are mononuclear cells? Circulating or tissue-resident mononuclear cells are activated by immune or complement complexes and produce a range of cytokines and chemokines… Continue reading
chromatin Continue reading...

What is chromatin? Definition, structure… 14th June 2022

Chromatin is a mass of genetic fabric composed of DNA and proteins that condenses to shape chromosomes all through eukaryotic mobileular department. Chromatin is placed withinside the nucleus of our cells. The primary function of this composed of DNA and proteins is to compress DNA into a compact unit that is smaller and can fit inside the nucleus. Chromatin consists of complexes of small proteins called histones and DNA. Histones assist organise DNA into systems… Continue reading
What is monkeypox Causes, Symptoms... Continue reading...

What is monkeypox? Causes, Symptoms… 21st May 2022

Monkeypox virus belongs to the genus Orthopoxvirus withinside the own circle of relatives Poxviridae. The genus Orthopoxvirus also includes smallpox virus (which causes smallpox), vaccinia virus (used in the smallpox vaccine) and bovine pox virus. Monkeypox is a rare disease caused by infection with the monkeypox virus. But while the transmissions have caused concern among government officials and infectious disease experts, and the Covid-wary world is on alert for any new outbreaks, there are several… Continue reading
Northern Blotting technique is used for Continue reading...

Northern Blotting technique is used for 8th May 2022

Northern blotting technique is a technique used to study gene expression. It is performed by the detection of a specific RNA (or isolated mRNA). The mRNA is usually represented in 5% of the total RNA sequence. This method reveals the identity, number, activity and size of the particular gene. Northern Blotting Technique This blotting technique can also be used for the growth of a tissue or organism. At different stages of differentiation and morphogenesis, the… Continue reading
Continue reading...

Tumor P53 Protein – p53 gene 20th March 2022

p53 gene, known as the "genome guard", is a gene that produces a protein located inside the nucleus of cells that plays a key role in controlling cell division and death. Mutations (changes) in the p53 gene can cause cancer cells to grow and spread throughout the body. These changes have been found in a genetic disease called Li-Fraumeni syndrome and in many types of cancer. This gene is a type of tumour suppressor gene.… Continue reading
gene knockout Continue reading...

Gene Knockout 2022

Gene knockout, also called gene inactivation, is a genetic technique in which the expression of a particular gene in an organism is suppressed by replacing the original gene at its genetic locus with a modified version of the gene in which one or more exons have been deleted, resulting in organisms that do not express the gene in a particular tissue or in the whole organism. These organisms are also called knockouts. A knockout is… Continue reading
Deadliest viruses in history Continue reading...

Deadliest viruses in history 25th February 2022

According to Peter Crosta (UoI), "viruses are microscopic organisms that exist almost everywhere on the planet. They can infect animals, plants, fungi and even bacteria... Humans have witnessed some of the deadliest viral pandemics, with far-reaching consequences. However, vaccines and antiviral drugs have helped to reduce the risk and spread of disease, as well as prevent serious illness and death. Vaccines help the body to safely develop immunity against a disease. There have been outbreaks… Continue reading
Southern Blotting Techniques Continue reading...

Southern Blotting Techniques 22nd February 2022

Southern blotting is a fundamental technique in molecular biology. It was first invented by E.M. Southern in 1975 as an important method for transferring DNA molecules, especially restriction fragments, from an electrophoresis gel to a nitrocellulose or nylon sheet, usually called a membrane. During the process, the DNA band pattern present in the gel is replicated on the membrane. After post-treatment, the DNA is immobilised on the membrane and can be used as a substrate… Continue reading
ras kits Continue reading...

Ras Kits 18th February 2022

Ras kits, antibodies and plasmids are high-quality, validated tools for research. The Ras, HRAS and KRAS genes were identified by Edward M. Scolnick and colleagues following the study of two cancer-causing viruses, Harvey sarcoma virus and Kirsten sarcoma virus. The viruses were first discovered in rats by Jennifer Harvey and Werner H. Kirsten, respectively, hence the name rat sarcoma. Later, in 1982, Geoffrey M. Cooper discovered human ras genes activated and transformed into human cancer… Continue reading
plasmid dna isolation Continue reading...

Plasmid DNA Isolation protocol, principle, steps… 16th February 2022

Isolation of plasmid DNA or "plasmid dna isolation" from micro organism is a critical approach in molecular biology and is an crucial step in lots of processes which includes cloning, DNA sequencing, transfection and gene therapy. These processes require the isolation of high purity plasmid DNA. The purified plasmid DNA can be used immediately in all molecular biology procedures such as restriction enzyme digestion, cloning, PCR, transfection, in vitro translation, blotting and sequencing. Steps for… Continue reading