(S)-nitro-Blebbistatin

(S)-nitro-Blebbistatin

• SMILES: O[C@]12C(N(CC1)C3=CC=CC=C3)=NC=4C(C2=O)=CC=C(N(=O)=O)C4
• Formula: C17H13N3O4

• Purity: 0.98
• CAS#: 856925-75-2
• Molecular Weight: 323.3
• Bioactivity: (S)-nitro-Blebbistatin is a more stable form of (-)-blebbistatin , which is a selective cell-permeable inhibitor of non-muscle myosin II ATPases. (-)-Blebbistatin rapidly and reversibly inhibits Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB for several species (IC50s = 0.5-5 μM), while poorly inhibiting smooth muscle myosin (IC50 = 80 μM). Through these effects, it blocks apoptosis-related bleb formation, directed cell migration, and cytokinesis in vertebrate cells. However, prolonged exposure to blue light (450-490 nm) results in degradation of blebbistatin to an inactive product via cytotoxic intermediates, which may be problematic for its use in fluorescent live cell imaging applications. The addition of a nitro group stabilizes the molecule to circumvent its degradation by prolonged blue light exposure. (S)-nitro-Blebbistatin has the same stereochemistry as the active (-)-blebbistatin enantiomer.

For research use only.