Product Overview
_x000D_The Multiplex ELISA Kit For Mouse Cytokine Panel 3 (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IL-2, IL-4, IL-6, TNFα).
Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing four distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Using just 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 5.5 hours. Multiplex ELISA Kit For Mouse Cytokine Panel 3 (4-Plex) provides scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.
_x000D__x000D_Assay Ranges
_x000D_| Analyte | Assay Type | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection |
|---|---|---|---|---|---|
| IL-2 | Sandwich | 360 - 0.49 (pg/mL) | 252 (pg/mL) | 0.6 (pg/mL) | 0.25 (pg/mL) |
| IL-4 | Sandwich | 400 - 0.55 (pg/mL) | 280 (pg/mL) | 0.7 (pg/mL) | 0.27 (pg/mL) |
| IL-6 | Sandwich | 504 - 0.69 (pg/mL) | 353 (pg/mL) | 0.91 (pg/mL) | 0.35 (pg/mL) |
| TNFα | Sandwich | 429 - 0.59 (pg/mL) | 300 (pg/mL) | 0.69 (pg/mL) | 0.29 (pg/mL) |
Kit Components
_x000D_Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.
_x000D__x000D_Reagents Include:
_x000D_Calibrator: Lyophilized, recombinant antigens in a buffered protein base;_x000D_Detection Mix: Lyophilized, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives;_x000D_Sample Diluent: Liquid, 10 mL/vial of a buffered protein solution;_x000D_Substrate A: Liquid, 3.5 mL/vial;_x000D_Substrate B+: Liquid, 3.5 mL/vial;_x000D_Streptavidin-HRP: Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase;_x000D_Wash Buffer Concentrate (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant;_x000D_Plate Seals (3): Adhesive strips
The Multiplex ELISA Kit For Mouse Cytokine Panel 3 (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IL-2, IL-4, IL-6, TNFα).
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