IL-8 is a proinflammatory CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is secreted by monocytes and endothelial cells. IL-8 chemoattracts and activates neutrophils. Recombinant human IL-8 (monocyte-derived) is an 8.4 kDa protein containing 72 amino acid residues.
- Gene name: IL-8 (72aa) (CXCL8)
- Host: E. coli
- Label: None
- Applications: Determined by its ability to chemoattract human peripheral blood neutrophils using a concentration range of 10.0-100.0 ng/ml.
- Species Reactivity/Origin species: Monkey, Mouse, Rat, Human, Bacteria
- Purity: > 98% by SDS-PAGE & HPLC analyses
- Endotoxin levels: < 0.1 ng/µg of protein (<1EU/µg)
- Reconstitution Buffer: water
- Additives/Stabilizer: None
- Form: lyophilized
- Storage: The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.
- Reconstitution tips: Centrifuge the vial prior for opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
- MW: 8.4 kDa
- Length (aa): 72
- Protein Sequence: SAKELRCQCI KTYSKPFHPK FIKELRVIES GPHCANTEII VKLSDGRELC LDPKENWVQR VVEKFLKRAE NS
- NCBI GeneID: 3576
- Accession Number Protein: NP_000575.1
- Accession Numberm RNA: NM_000584.3
- Uniprot: P10145
- Chromosomal location: 4q13-q21
For research use only.
0 reviews for ReliaTech