A competitive ELISA kit for quantitative measurement of 12-HETE (12-Hydroxyeicosatetraenoic Acid) in samples from Serum, Plasma, Cell supernatant
Gentaur's 12-HETE ELISA kit utilizes the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 12-HETE. During the reaction, 12-HETE in the sample or standard competes with a fixed amount of 12-HETE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 12-HETE. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 12-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.
The kit is shipped on ice packs or blue ice. Upon receving it, keep refrigerated at 1 to 8 degress Celsius. The shelf life of this is guaranteed to be at least 6 months from the date of receipt.
- Species reactivity: Universal
- Antigen: 12-HETE
- Assay type: Competitive ELISA
- Sensitivity: 0.47ng/mL
- Detections range: from: 0.78 to: 50ng/mL
- Validated sample types: Serum, Plasma, Cell supernatant
This Universal 12-HETE ELISA kit is intended for research use only. Do not use for human or animal diagnostics.
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