DAPK2 Antibody: Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNγ induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1. Ectopicly expressed DAPK2 induced apoptosis in various types of cells. DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues.
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
- DAPK2 antibody can be stored at 4˚
- C for three months and -20˚
- C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Research area: Apoptosis, Autophagy
- Host species: Rabbit
- Species reactivity: Human, Mouse, Rat
- Homology: N/A
- Immunogen: DAPK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DAPK2.
- This antigenic peptide is identical to the corresponding amino acids of mouse origin (1,2).
- The immunogen is located within the last 50 amino acids of DAPK2.
- Tested Applications: E, WB, IHC-P
- Applications: DAPK2 antibody can be used for detection of DAPK2 by Western blot at 1 μ
- g/mL. An approximately 42 kDa band can be detected. DAPK2 has no cross responses to DAPK1. Antibody can also be used for immunohistochemistry starting at 2 μ
- g/mL.
- Antibody validated: Western Blot in human, mouse, and rat samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
- Specificity: DAPK2 has no cross responses to DAPK1.
- Positive control 1: Cat. No. 1202 - A431 Cell Lysate
- Positive control 2: Cat. No. 1406 - Mouse Spleen Tissue Lysate
- Positive control 3: Cat. No. 1465 - Rat Kidney Tissue Lysate
- Positive control 4: N/A
- Positive control 5: N/A
- Positive control 6: N/A
- Predicted molecular weight: 42 kDa
- MW explanation: N/A
- Validation: N/A
- Isoforms: N/A
- Purification: DAPK2 Antibody is affinity chromatography purified via peptide column.
- Clonality: Polyclonal
- Clone: N/A
- Isotype: IgG
- Conjugate: Unconjugated
- Physical state: Liquid
- Buffer: DAPK2 Antibody is supplied in PBS containing 0.02% sodium azide.
- Concentration: 1 mg/ml
- NCBI official symbol: DAPK2
- Accession #: BAA88063
- Protein GI #: 6521210
- NCBI gene ID#: 23604
- NCBI official full name: death-associated protein kinase 2
- NCBI organism: Homo sapiens
- SWISSPROT #: Q9UIK4
Optimal dilutions for each application to be determined by the researcher.
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