Bit1 Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apoptosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
- Bit1 antibody can be stored at 4˚
- C for three months and -20˚
- C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Research area: Apoptosis
- Host species: Rabbit
- Species reactivity: Human, Mouse, Rat
- Homology: N/A
- Immunogen: Bit1 antibody was raised against a 15 amino acid synthetic peptide from near the amino-terminus of human Bit1.
- The immunogen is located within amino acids 20 - 70 of Bit1.
- Tested Applications: E, WB, ICC, IF
- Applications: Bit1 antibody can be used for the detection of Bit1 by Western blot at 1 - 4 μ
- g/mL. Antibody can also be used for immunocytochemistry starting at 2 μ
- g/mL. For immunofluorescence start at 20 μ
- g/mL.
- Antibody validated: Western Blot in mouse samples
- Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
- Specificity: N/A
- Positive control 1: Cat. No. 1288 - A20 Cell Lysate
- Positive control 2: Cat. No. 1212 - 3T3 Cell Lysate
- Positive control 3: Cat. No. 17-201 - 3T3/BALB Cell Slide
- Positive control 4: N/A
- Positive control 5: N/A
- Positive control 6: N/A
- Predicted molecular weight: N/A
- MW explanation: N/A
- Validation: N/A
- Isoforms: N/A
- Purification: Bit1 Antibody is affinity chromatography purified via peptide column.
- Clonality: Polyclonal
- Clone: N/A
- Isotype: IgG
- Conjugate: Unconjugated
- Physical state: Liquid
- Buffer: Bit1 Antibody is supplied in PBS containing 0.02% sodium azide.
- Concentration: 1 mg/mL
- NCBI official symbol: PTRH2
- Accession #: NP_057161
- Protein GI #: 7706351
- NCBI gene ID#: 51651
- NCBI official full name: peptidyl-tRNA hydrolase 2
- NCBI organism: Homo sapiens
- SWISSPROT #: Q9Y3E5
Optimal dilutions for each application to be determined by the researcher.
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