ELISA Enzyme linked Immunosorbent Assay Sandwich Continue reading...

Guide ELISA (Enzyme-linked Immunosorbent Assay) 23rd November 2021

¿What is a Enzyme-linked Immunosorbent Assay (ELISA)? ELISA (Enzyme-linked Immunosorbent Assay) is a plate-based assay technique designed to detect and quantify soluble substances such as peptides, proteins, antibodies and hormones. Other names, such as enzyme immunoassay (EIA), are also used to describe the same technology. In an ELISA, the antigen (target macromolecule) is immobilized on a solid surface (microplate) and then forms a complex with an antibody that is bound to a reporter enzyme. Detection… Continue reading
ELISA Continue reading...

ELISA (Enzyme-linked immunosorbent assay) 9th November 2021

Introduction Enzyme-linked immunosorbent assay (ELISA) is a powerful method to detect and quantify a specific protein in a complex mixture. Originally described by Engvall and Perlmann (1971), the method allows the analysis of protein samples immobilised in microplate wells using specific antibodies. ELISAs are usually performed in 96- or 384-well polystyrene plates, which passively bind antibodies and proteins. This binding and immobilisation of reagents is what makes ELISAs easy to design and perform. The immobilisation… Continue reading