TurboTEV Protease contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. TurboTEV Protease is a restriction grade protease that has a robust activity at 4°C with high specificity and great stability. It does not require any special buffer for its activity and can be used in a buffer most suitable for the target protein. TurboTEV Protease is a 52 kDa protein with both GST and His-tags so it can be easily removed by either Ni-chelating or Glutathione (GSH) resin along with the cleaved tag. In practice, 1 mg (10,000 units) of TurboTEV Protease cleaves >90% of 100 mg of a control target protein at 4°C in 16 hours. No non-specific cleavage has been observed under the same condition when TurboTEV Protease and the control target protein was mixed at 1:10 ratio.
TurboTEV Protease
BPS BioscienceSupplier: Gentaur
Inc. VAT:940.96 GBP
Catalog: 421-50308
Product Size: 1000 units
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