G9a Chemiluminescent Assay Kit

The G9a Direct Activity Chemiluminescent Assay Kit is designed to measure G9a_x000D_activity for screening and profiling applications. The key to the G9a Direct Activity Assay_x000D_Kit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. With_x000D_this kit, only three simple steps are required for methyltransferase detection. First,_x000D_S-adenosylmethionine is incubated with a sample containing assay buffer_x000D_and methyltransferase enzyme for one hour. Next, primary antibody is added._x000D_Finally, the strips are treated with an HRP-labeled secondary antibody followed by_x000D_addition of the HRP substrate to produce chemiluminescence that can then be measured_x000D_using a chemiluminescence reader.