FRMPD2 Antibody: The FERM and PDZ domain containing (FRMPD) protein family consists of four proteins that contain a FERM (Four-point-one, erzin, radixin, moesin) domain and at least one PDZ (PSD-95/Discs large/Zonula-occuldens-1) domain. FRMPD2 also contains an N-terminal KIND domain and three PDZ domains and is structurally similar to the protein tyrosine phosphatase PTP-BL. FRMPD2 is localized in a polarized fashion in epithelial cells at the basolateral membrane and partially co-localizes with the tight-junction marker protein Zonula-occuldens-1. Suppression of FRMPD2 expression via RNAi in Caco-2 cells results in an impairment of tight junction formation, indicating that FRMPD2 plays a major role in tight junction formation. Other experiments indicate that FRMPD2 is a binding partner to several catenin family members and recruitment of FRMPD2 to cell-cell contacts is dependent on E-cadherin-mediated cell-cell adhesion.
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
- FRMPD2 antibody can be stored at 4˚
- C for three months and -20˚
- C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Research area: Homeostasis
- Host species: Rabbit
- Species reactivity: Human, Mouse, Rat
- Homology: N/A
- Immunogen: FRMPD2 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human FRMPD2.
- The immunogen is located within amino acids 80 - 130 of FRMPD2.
- Tested Applications: E, WB, IHC-P
- Applications: FRMPD2 antibody can be used for detection of FRMPD2 by Western blot at 1 - 2 μ
- g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ
- g/mL.
- Antibody validated: Western Blot in rat samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
- Specificity: N/A
- Positive control 1: Cat. No. 1465 - Rat Kidney Tissue Lysate
- Positive control 2: N/A
- Positive control 3: N/A
- Positive control 4: N/A
- Positive control 5: N/A
- Positive control 6: N/A
- Predicted molecular weight: N/A
- MW explanation: N/A
- Validation: N/A
- Isoforms: N/A
- Purification: FRMPD2 Antibody is affinity chromatography purified via peptide column.
- Clonality: Polyclonal
- Clone: N/A
- Isotype: IgG
- Conjugate: Unconjugated
- Physical state: Liquid
- Buffer: FRMPD2 Antibody is supplied in PBS containing 0.02% sodium azide.
- Concentration: 1 mg/mL
- NCBI official symbol: FRMPD2
- Accession #: NP_001018081
- Protein GI #: 281604114
- NCBI gene ID#: 143162
- NCBI official full name: FERM and PDZ domain containing 2
- NCBI organism: Homo sapiens
- SWISSPROT #: Q68DX3
Optimal dilutions for each application to be determined by the researcher.
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