Human Shh cDNA encodes a 462 amino acid (aa) residue (45 kDa) precursor protein with a 23 aa signal peptide. An autocatalytic cleavage reaction yields a 19 kDa (residues 24 - 197) amino-terminal fragment (Shh-N), and a 25 kDa (residues 198 - 462) carboxy-terminal domain (Shh-C). The N-terminal domain retains all known signaling capabilities, while the C-terminal domain is responsible for the intramolecular processing, acting as a cholesterol transferase that covalently transfers the cholesterol molecule to the C-terminus of Shh-N. When Shh is expressed in insect or mammalian cells, a palmitoyl group is also attached to the N-terminal cysteine of Shh-N via an amide linkage.
- Gene name: Sonic Hedgehog (Shh) C-terminal
- Host: Rat
- Label: None
- Clone: (#4A37)
- Isotype: IgG2
- Applications: WB
- Species Reactivity/Origin species: Mouse
- Antigen: recombinant mouse Shh (C-terminal) fragment
- Purification method: Protein G chromatography
- Reconstitution Buffer: PBS
- Buffer: PBS
- Additives/Stabilizer: None
- Form: lyophilized
- Storage: Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.
- Reconstitution tips: Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
- NCBI GeneID: 20423
- Accession Number Protein: NP_033196.1
- Accession Numberm RNA: NM_009170.3
- Uniprot: Q62226
- Chromosomal location: 5 B1
- 5 16.0 cM
For research use only.
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