PHF8 (KDM7B) Homogeneous Assay Kit

The PHF8 Homogeneous Assay Kit is designed to measure the activity of_x000D_PHF8 for screening and profiling applications. PHF8, also known as JHDM1F and KDM7B, is_x000D_a JumonjiC (JmjC) domain-containing histone lysine demethylase that exhibits demethylation_x000D_activity toward dimethyl-lysine 9 on histone H3. The PHF8 Homogeneous Assay Kit comes in a_x000D_convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody,_x000D_demethylase assay buffer, and purified PHF8 for 384 enzyme reactions. The key to the PHF8_x000D_Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate._x000D_With this kit, only three simple steps on a microtiter plate are required for demethylase activity_x000D_detection. First, a sample containing PHF8 enzyme is incubated with the biotinylated substrate._x000D_Next, acceptor beads and primary antibody are added, then donor beads, followed by reading_x000D_the Alpha-counts.