ATG13 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG13 forms a complex with ULK1 and ULK2, the mammalian homologs of ATG1, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. Knockdown of ATG13 inhibits autophagosome formation.
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
- ATG13 antibody can be stored at 4˚
- C for three months and -20˚
- C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Research area: Autophagy
- Host species: Rabbit
- Species reactivity: Human, Mouse, Rat
- Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)
- Immunogen: ATG13 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ATG13.
- The immunogen is located within amino acids 450 - 500 of ATG13.
- Tested Applications: E, WB, IHC-P, IF
- Applications: ATG13 antibody can be used for detection of ATG13 by Western blot at 1 μ
- g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ
- g/mL. For immunofluorescence start at 20 μ
- g/mL.
- Antibody validated: Western Blot in rat samples
- Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
- Specificity: Multiple isoforms of ATG13 are known to exist.
- Positive control 1: Cat. No. 1461 - Rat Heart Tissue Lysate
- Positive control 2: N/A
- Positive control 3: N/A
- Positive control 4: N/A
- Positive control 5: N/A
- Positive control 6: N/A
- Predicted molecular weight: Predicted: 44, 53, 57, 61 kDa
- Observed: 49 kDa
- MW explanation: N/A
- Validation: N/A
- Isoforms: N/A
- Purification: ATG13 Antibody is affinity chromatography purified via peptide column.
- Clonality: Polyclonal
- Clone: N/A
- Isotype: IgG
- Conjugate: Unconjugated
- Physical state: Liquid
- Buffer: ATG13 Antibody is supplied in PBS containing 0.02% sodium azide.
- Concentration: 1 mg/mL
- NCBI official symbol: ATG13
- Accession #: NP_001136145
- Protein GI #: 218082953
- NCBI gene ID#: 9776
- NCBI official full name: ATG13 autophagy related 13 homolog (S. cerevisiae)
- NCBI organism: Homo sapiens
- SWISSPROT #: O75143
Optimal dilutions for each application to be determined by the researcher.
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