Aipl1 Antibody: Aipl1 was initially identified as a protein implicated in Leber congenital amaurosis (LCA), an autosomal recessive disorder thought to be caused by the abnormal development of photoreceptors. Aipl1 is a tetratricopeptide repeat protein that is highly homologous to ARA9, a protein involved in the HSP90-mediated nuclear translocation and transactivation of the aryl hydrocarbon receptor. Aipl1 has also been found to function as part of a chaperone heterocomplex, interacting with Hsp90 and Hsp70. Aipl1 also associates with the cell cycle regulator NUB1. It is thought that Aipl1 cooperates with Hsp70 but not Hsp90 to suppress the formation of NUB1 inclusions, and these interactions are necessary in the normal photoreceptor maturation, as mutations that lead to LCA also compromise the interactions with the Hsp chaperones. At least three isoforms of Aipl1 are known to exist.
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
- Aipl1 antibody can be stored at 4˚
- C for three months and -20˚
- C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Research area: Homeostasis
- Host species: Rabbit
- Species reactivity: Human, Mouse, Rat
- Homology: Predicted species reactivity based on immunogen sequence: Bovine: (78%)
- Immunogen: Aipl1 antibody was raised against a 18 amino acid synthetic peptide near the center of the human Aipl1.
- The immunogen is located within amino acids 140 - 190 of Aipl1.
- Tested Applications: E, WB, IHC-P, IF
- Applications: Aipl1 antibody can be used for detection of Aipl1 by Western blot at 1 - 2 μ
- g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ
- g/mL. For immunofluorescence start at 20 μ
- g/mL.
- Antibody validated: Western Blot in rat samples
- Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- Specificity: N/A
- Positive control 1: Cat. No. 1463 - Rat Brain Tissue Lysate
- Positive control 2: Cat. No. 10-301 - Human Brain Tissue Slide
- Positive control 3: N/A
- Positive control 4: N/A
- Positive control 5: N/A
- Positive control 6: N/A
- Predicted molecular weight: N/A
- MW explanation: N/A
- Validation: N/A
- Isoforms: N/A
- Purification: Aipl1 Antibody is affinity chromatography purified via peptide column.
- Clonality: Polyclonal
- Clone: N/A
- Isotype: IgG
- Conjugate: Unconjugated
- Physical state: Liquid
- Buffer: Aipl1 Antibody is supplied in PBS containing 0.02% sodium azide.
- Concentration: 1 mg/mL
- NCBI official symbol: AIPL1
- Accession #: NP_055151
- Protein GI #: 74272276
- NCBI gene ID#: 23746
- NCBI official full name: aryl hydrocarbon receptor interacting protein-like 1
- NCBI organism: Homo sapiens
- SWISSPROT #: Q9NZN9
Optimal dilutions for each application to be determined by the researcher.
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