Alternative Names/Synonyms: Ac-DEVD-AFC, Caspase-3 Substrate Fluorogenic
Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7 uM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
Appearance: Liquid
Formulation: In DMSO (1 mM)
CAS Number: N/A
Structure Available: No
Peptide sequence: Ac-Asp-Glu-Val-Asp-AFC
Salt Form: No
Molecular Formula: C30H33F3N5O13
Molecular Weight: 729
Cell-Permeable: No
Purity: >98% by HPLC
Solubility/Reconstitution Instructions: N/A
Shipping Conditions: gel pack
Storage Temperature: -20 Celsius
Handling: Potect from light and moisture
Shelf life: 6-12 months
MDL Number: MFCD01310970
PubChem CID: 644234
PubChem CID Link: Click Here
Category: Caspase Substrate
MSDS Available: yes
Key Words: Caspase-3 Substrate, Caspase-3 Substrate, fluorogenic, Caspase3 Substrate, fluorogenic, Caspase3 Substrate, Caspase 3 Substrate, DEVD-AFC, caspase-3 assay, DEVDase substrate
For more information, please, refer to the full datasheet.