Southern Blotting Techniques Continue reading...

Southern Blotting Techniques 22nd February 2022

Southern blotting is a fundamental technique in molecular biology. It was first invented by E.M. Southern in 1975 as an important method for transferring DNA molecules, especially restriction fragments, from an electrophoresis gel to a nitrocellulose or nylon sheet, usually called a membrane. During the process, the DNA band pattern present in the gel is replicated on the membrane. After post-treatment, the DNA is immobilised on the membrane and can be used as a substrate… Continue reading
Southern Blotting Continue reading...

Southern Blotting 27th October 2021

Southern BlottingIntroduction Southern Blotting or Southern blot es un método de Molecular biology which detects the presence of a particular DNA sequence in a complex mixture of this nucleic acid. Southern blotting is used in molecular biology for the identification of proteins and nucleic acids and is widely used for diagnostic purposes. This technique immobilises the molecule of interest on a support, which is a nitrocellulose or nylon membrane. It uses hybridisation techniques for the identification of nucleic… Continue reading