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Standard PCR protocol , how to do PCR (Polymerase Chain Reaction) 15th November 2021

Introduction This article will discuss the PCR protocol in depth to provide clarity on this topic. Once you have your genetic samples, one of the first steps in PCR is to design the primers needed to perform the PCR reaction. The first step in proceeding with a standard PCR is to design the primers. You will need to determine which fragment of the DNA template you want to amplify, for which you will need to… Continue reading
Polymerase Chain Reaction ASO PCR Continue reading...

Polymerase chain reaction (ASO-PCR) 18th October 2021

ASO-PCR (alternative method for mutation detection)Introduction Allele-specific oligonucleotide polymerase chain reaction (ASO-PCR) is an alternative method for mutation detection. The advantage of ASO-PCR is that it is a fast, simple and non-radioactive method. Increasingly, many single base pair substitutions are being discovered that lead to inherited diseases, predisposition to genetic disorders and cancer. The ability to amplify specific DNA sequences by polymerase chain reaction (PCR) has made it possible to quickly and accurately diagnose many… Continue reading