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Standard PCR protocol , how to do PCR (Polymerase Chain Reaction) 15th November 2021

Introduction This article will discuss the PCR protocol in depth to provide clarity on this topic. Once you have your genetic samples, one of the first steps in PCR is to design the primers needed to perform the PCR reaction. The first step in proceeding with a standard PCR is to design the primers. You will need to determine which fragment of the DNA template you want to amplify, for which you will need to… Continue reading
Polymerase Chain Reaction ASO PCR Continue reading...

Polymerase chain reaction (ASO-PCR) 18th October 2021

ASO-PCR (alternative method for mutation detection)Introduction Allele-specific oligonucleotide polymerase chain reaction (ASO-PCR) is an alternative method for mutation detection. The advantage of ASO-PCR is that it is a fast, simple and non-radioactive method. Increasingly, many single base pair substitutions are being discovered that lead to inherited diseases, predisposition to genetic disorders and cancer. The ability to amplify specific DNA sequences by polymerase chain reaction (PCR) has made it possible to quickly and accurately diagnose many… Continue reading
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Moderna The History of the Company 17th October 2021

History of Moderna Company Inc (mRNA)Introduction Moderna, Inc. is a US biotechnology and pharmaceutical company based in Cambridge, Massachusetts. It focuses on messenger RNA (mRNA)-based vaccine technologies. Moderna's vaccine platform inserts synthetic nucleoside-modified messenger RNA (modRNA) into human cells using a layer of lipid nanoparticles. This mRNA then reprograms the cells to elicit immune responses. Moderna develops therapeutic mRNA vaccines that are delivered in lipid nanoparticles, using mRNA with Pseudouridine nucleosides. Candidates are designed to… Continue reading
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GeneXpert® Systems 10th August 2021

We present to You Systems from GeneXpert® made by Cepheid®. What are GenExpert® Systems? Genexpert Systems are instruments that may improve diagnostic research. The focus of their work is real time PCR. Other name for this technique is quantitative PCR. It allows us to determine the amount of a given DNA sequence in the sample by means of fluorescence measurements. In this kind of PCR mRNA is a template. DNA synthesis on the mRNA template… Continue reading
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FilmArray® multiplex PCR system 4th August 2021

We present to You FilmArray® PCR systems from BioFire®. What is FilmArray® System? BioFire® system devices use multiplex PCR technology. They are produced to do work of several different instruments at once. They take samples, extract genetic material, amplificate, detect and analize the results of experiment. They are really fast, because it takes them aproximately 2 minutes to take a sample and less than an hour to give results. They enable to detect simultaneously bacteria,… Continue reading
Pipette filter tips - high demand, low supply and uncertain future for the PCR tests Continue reading...

Pipette filter tips – high demand, low supply and uncertain future for the PCR tests 6th November 2020 – Posted in: Virus

Due to the increased use of PCR there is a worldwide shortage of 1000ul and 1250ul PCR filter tips that are used for safe handling of DNA and RNA samples. Big distributors like Gentaur had already built up a filter tip stock to continue supplying filter tips to diagnostic and forensic laboratories but this stock is already lacking the large filter tips. Small filter tips are still available. Apparently this will not be sufficient since… Continue reading