Southern blotting is a fundamental technique in molecular biology. It was first invented by E.M. Southern in 1975 as an important method for transferring DNA molecules, especially restriction fragments, from an electrophoresis gel to a nitrocellulose or nylon sheet, usually called a membrane. During the process, the DNA band pattern present in the gel is... Continue reading
Western blotting, or immunoblotting, is an analytical technique used in cell and molecular biology to identify specific proteins in a complex mixture of proteins, such as those present in cell or tissue extracts. The technique uses three steps to achieve this: separation by size, transfer to a solid medium and, finally, visualisation. by protein tagging... Continue reading
