Southern Blotting Techniques Continue reading...

Southern Blotting Techniques 22nd February 2022

Southern blotting is a fundamental technique in molecular biology. It was first invented by E.M. Southern in 1975 as an important method for transferring DNA molecules, especially restriction fragments, from an electrophoresis gel to a nitrocellulose or nylon sheet, usually called a membrane. During the process, the DNA band pattern present in the gel is replicated on the membrane. After post-treatment, the DNA is immobilised on the membrane and can be used as a substrate… Continue reading
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Western blot (Immunoblot) : Gel electrophoresis of proteins 25th October 2021

Western blot (Immunoblot) : Gel electrophoresis of proteins Western blotting, or immunoblotting, is an analytical technique used in cell and molecular biology to identify specific proteins in a complex mixture of proteins, such as those present in cell or tissue extracts. The technique uses three steps to achieve this: separation by size, transfer to a solid medium and, finally, visualisation. by protein tagging with the use of appropriate primary or secondary antibodies. Western blot What… Continue reading