chromatin Continue reading...

What is chromatin? Definition, structure… 14th June 2022

Chromatin is a mass of genetic fabric composed of DNA and proteins that condenses to shape chromosomes all through eukaryotic mobileular department. Chromatin is placed withinside the nucleus of our cells. The primary function of this composed of DNA and proteins is to compress DNA into a compact unit that is smaller and can fit inside the nucleus. Chromatin consists of complexes of small proteins called histones and DNA. Histones assist organise DNA into systems… Continue reading
plasmid dna isolation Continue reading...

Plasmid DNA Isolation protocol, principle, steps… 16th February 2022

Isolation of plasmid DNA or "plasmid dna isolation" from micro organism is a critical approach in molecular biology and is an crucial step in lots of processes which includes cloning, DNA sequencing, transfection and gene therapy. These processes require the isolation of high purity plasmid DNA. The purified plasmid DNA can be used immediately in all molecular biology procedures such as restriction enzyme digestion, cloning, PCR, transfection, in vitro translation, blotting and sequencing. Steps for… Continue reading
Complementary DNA cDNA Continue reading...

Complementary DNA (cDNA) 12th February 2022

Complementary DNA ( cDNA ) is a double-stranded DNA model of an mRNA molecule. In higher eukaryotes, an mRNA is a more useful predictor of a polypeptide sequence than a genomic sequence, because the introns have been spliced together. Researchers prefer to use cDNA rather than mRNA itself because RNAs are inherently less stable than DNA and there are no techniques to routinely amplify and purify individual RNA molecules. Complementary DNA is crafted from mRNA… Continue reading
gene definition Continue reading...

What is a gene? A complete guide to genes 28th November 2021

Introduction We are all familiar with genetics or gene, which is made up of DNA. Many young people will have first learned about genetics and DNA from the legendary film Jurassic Park, which talked about DNA and genetics to create dinosaurs. However, humans also have DNA, and for us, it can influence everything around us. Everyone has DNA, and therefore everyone has genes. Genes are the basic physical and functional units of heredity, they are… Continue reading
Recombinant DNA technology Continue reading...

Recombinant DNA technology 19th November 2021

Recombinant DNA technologyIntroduction Recombinant DNA technology is playing a key role in improving health conditions through the development of new vaccines and pharmaceuticals. Treatment strategies are also improved through the development of diagnostic kits, monitoring devices and new therapeutic approaches. The synthesis of synthetic human insulin and erythropoietin using genetically modified bacteria and the production of new types of experimental mutant mice for research purposes are among the main examples of genetic engineering in the… Continue reading
Branched DNA Continue reading...

Branched DNA technology (bDNA) 14th November 2021

Introduction The Branched DNA Assay (bDNA) provides a unique and powerful tool for the reliable quantification of nucleic acid molecules. Fundamentally different from target amplification methods such as PCR, the bDNA assay directly measures nucleic acid molecules at physiological levels by amplifying the reporter signal, rather than replicating the target sequences as a means of detection, and thus avoids the errors inherent in the extraction and amplification of target sequences. The bDNA assay employs linear… Continue reading