Polymerase chain reaction (ASO-PCR) 18th October 2021

ASO-PCR (alternative method for mutation detection)Introduction Allele-specific oligonucleotide polymerase chain reaction (ASO-PCR) is an alternative method for mutation detection. The advantage of ASO-PCR is that it is a fast, simple and non-radioactive method. Increasingly, many single base pair substitutions are being discovered that lead to inherited diseases, predisposition to genetic disorders and cancer. The ability to amplify specific DNA sequences by polymerase chain reaction (PCR) has made it possible to quickly and accurately diagnose many… Continue reading