Human uterus-fundus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-fundus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-fundus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-fundus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
- Lysate Type: Tissue
- Tested Applications: WB
- Applications: Uterus: Fundus membrane protein lysate is for use in Western blotting, 10 μ
- g to 20 μ
- g per lane is recommended for mini gel.
- Physical State: Liquid
- Buffer: HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
- Concentration: 2 mg/mL or better
- Source: Human Uterus: Fundus tissue
- Tissue by diagnosis: Normal
- Tissue by development stage: N/A
- Tissue by species: Human
- Tissue cell/type: Uterus: Fundus
- Diagnosis: Normal
- Cancer type: N/A
- Cancer extended: N/A
- Age: N/A
- Sex: N/A
- Race: N/A
- Species: Human
- Growth Media: N/A
This product is for research use only.
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