ULK2 Antibody

ULK2 Antibody: ULK2, also known as ATG1B, is a key serine/threonine protein kinase probably acting at the most upstream step of autophagosome formation. Knockout of ULK2 results in a severe defect in the autophagy pathway. ULK2 is highly conserved among eukaryotes and shows high homology with its related protein ULK1. Both ULK1 and ULK2 form a complex with ATG13 and FIP200 that mediates TOR signaling and is essential for autophagy. Like ULK1, ULK2 is also thought to be involved in early neuronal growth and differentiation.

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

• ULK2 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year.

• Research area: Autophagy
• Host species: Rabbit
• Species reactivity: Human
• Homology: Predicted species reactivity based on immunogen sequence: Mouse: (82%)
• Immunogen: ULK2 antibody was raised against a 17 amino acid peptide near the center of human ULK2 .
• The immunogen is located within amino acids 480 - 530 of ULK2.
• Tested Applications: E, WB, IHC-P, IF
• Applications: ULK2 Antibody can be used for detection of ULK2 by Western blot at 1 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: At least two isoforms of ULK2 are known to exist
• this antibody will detect both isoforms. ULK2 antibody is predicted to not cross-react with ULK1.
• Positive control 1: Cat. No. 1303 - Human Brain Tissue Lysate
• Positive control 2: Cat. No. 10-301 - Human Brain Tissue Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 105, 114 kDa
• Observed: 100 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: ULK2 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: ULK2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: ULK2
• Accession #: NP_055498
• Protein GI #: 217330557
• NCBI gene ID#: 9706
• NCBI official full name: unc-51-like kinase 2 (C. elegans)
• NCBI organism: homo sapiens
• SWISSPROT #: Q8IYT8

Optimal dilutions for each application to be determined by the researcher.