Survivin Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel IAP protein was recently identified and designated survivin, apoptosis inhibitor 4 (API4), and TIAP. Survivin/TIAP interacted with the processed form of caspase-3 and inhibited its proteolytic activity. Survivin/TIAP is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas.
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
- Survivin antibody can be stored at 4˚
- C for three months and -20˚
- C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Research area: Apoptosis, Cancer
- Host species: Rabbit
- Species reactivity: Human
- Homology: Predicted species reactivity based on immunogen sequence: Pig: (92%), Bovine: (79%), Mouse: (71%), Rat: (71%)
- Immunogen: Anti-Survivin antibody (2235) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Survivin.
- The immunogen is located within the last 50 amino acids of Survivin.
- Tested Applications: E, WB, ICC, IF
- Applications: WB: 1-5 μ
- g/mL
- ICC: 10 μ
- g/mL
- IF: 10 μ
- g/mL.
- Antibody validated: Western Blot in human samples
- Immunocytochemistry in human samples
- Immunofluorescence in human samples. All other applications and species not yet tested.
- Specificity: N/A
- Positive control 1: Cat. No. 1206 - MOLT4 Cell Lysate
- Positive control 2: Cat. No. 17-006 - MOLT-4 Cell Slide
- Positive control 3: Cat. No. 1201 - HeLa Cell Lysate
- Positive control 4: Cat. No. 1204 - K562 Cell Lysate
- Positive control 5: Cat. No. 1224 - Daudi Cell Lysate
- Positive control 6: N/A
- Predicted molecular weight: Predicted: 17kD
- Observed: 17 kD
- MW explanation: N/A
- Validation:
Independent Antibody Validation in Cell lines (Figure 2) shows similar Survivin expression profile in human cell lines detected by two independent anti-Survivin antibodies that recognize different epitopes, 2233 against N-terminus domain and 2235 against C-terminus domain.
- Survivin proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.
Recombinant Protein Test (Figure 6- 8): Anti-Survivin antibodies (2235) detected human Survivin recombinant protein directly or in sera from cancer patients.
- Isoforms: Human Survivin has 7 isoforms, including isoform 1 (142aa, 16kD), isoform 2 (165aa, 19kD), isoform 3 (137aa, 16kD), isoform 4 (120aa, 14kD), isoform 5 (117aa, 14kD), isoform 6 (78aa, 9kD) and isoform 7 (74aa, 8.5kD). Mouse Survivin has 3 isoforms, including isoform 1 (140aa, 16kD), isoform 2 (121aa, 14kD) and isoform 3 (40aa, 4.7kD). Rat Survivin has one isoform (142aa, 17kD). 2235 can detect human and possibly mouse and rat.
- Purification: Survivin Antibody is affinity chromatography purified via peptide column.
- Clonality: Polyclonal
- Clone: N/A
- Isotype: IgG
- Conjugate: Unconjugated
- Physical state: Liquid
- Buffer: Survivin Antibody is supplied in PBS containing 0.02% sodium azide.
- Concentration: 1 mg/ml
- NCBI official symbol: BIRC5
- Accession #: NP_001159
- Protein GI #: 59859878
- NCBI gene ID#: 332
- NCBI official full name: baculoviral IAP repeat-containing 5
- NCBI organism: Homo sapiens
- SWISSPROT #: O15392
Optimal dilutions for each application to be determined by the researcher.
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