StrepII-tag Antibody

StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

• StrepII-tag antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Research area: Other
• Host species: Rabbit
• Species reactivity: Tag
• Homology: N/A
• Immunogen: Anti-StrepII-tag antibody (4335) StrepII-tag antibody was raised against the amino acid sequence " srWSHPQFEKsh".
• Tested Applications: E, WB
• Applications: WB: 0.25-1 μ
• g/mL
• Antibody validated: StrepII-tag antibody can be used for the detection of StrepII-tagged proteins
• Specificity: N/A
• Positive control 1: N/A
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation:

  Recombinant Protein Test (Figure 1&

• 2): Anti-StrepII-tag antibodies (4335) detected StrepII-tagged recombinant protein at different concentrations.

• Isoforms: N/A
• Purification: StrepII-tag Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: StrepII-tag Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: N/A
• Accession #: N/A
• Protein GI #: N/A
• NCBI gene ID#: N/A
• NCBI official full name: N/A
• NCBI organism: N/A
• SWISSPROT #: N/A

Optimal dilutions for each application to be determined by the researcher.