SIK1 Antibody: Salt-inducible kinase 1 (SIK1), also known as SNF1LK or MSK, plays a role in histone modification and G2/M cell cycle regulation. It is a 783 amino acid protein that contains one UBA domain and belongs to the Ser/Thr protein kinase family (AMPK subfamily). Localized to both the nucleus and the cytoplasm, SIK1 is a class II HDAC kinase that uses magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins and is thought to be important for the early stages of skeletal muscle growth and myocardial cell differentiation.
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
- SIK1 antibody can be stored at 4˚
- C for three months and -20˚
- C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Research area: Signal Transduction
- Host species: Rabbit
- Species reactivity: Human
- Homology: Predicted species reactivity based on immunogen sequence: Rat: (73%), Mouse: (73%)
- Immunogen: SIK1 antibody was raised against an 18 amino acid synthetic peptide near the center of human SIK1.
- The immunogen is located within amino acids 340 - 390 of SIK1.
- Tested Applications: E, WB, IHC-P
- Applications: SIK1 antibody can be used for detection of SIK1 by Western blot at 1 μ
- g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ
- g/mL.
- Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
- Specificity: Two isoforms of SIK1 are known to exist
- this antibody will detect both isoforms. SIK1 antibody is predicted to not cross-react with other SIK protein family members.
- Positive control 1: Cat. No. 1308 - Human Small Intestine Tissue Lysate
- Positive control 2: Cat. No. 11-801 - Human Small Intestine Tissue Slide
- Positive control 3: N/A
- Positive control 4: N/A
- Positive control 5: N/A
- Positive control 6: N/A
- Predicted molecular weight: N/A
- MW explanation: N/A
- Validation: N/A
- Isoforms: N/A
- Purification: SIK1 Antibody is affinity chromatography purified via peptide column.
- Clonality: Polyclonal
- Clone: N/A
- Isotype: IgG
- Conjugate: Unconjugated
- Physical state: Liquid
- Buffer: SIK1 Antibody is supplied in PBS containing 0.02% sodium azide.
- Concentration: 1 mg/mL
- NCBI official symbol: SIK1
- Accession #: NP_775490
- Protein GI #: 116256471
- NCBI gene ID#: 150094
- NCBI official full name: salt-inducible kinase 1
- NCBI organism: Homo sapiens
- SWISSPROT #: P57059
Optimal dilutions for each application to be determined by the researcher.
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