Phospho-MSK1 Antibody

Product Quantity: 100 ug Manufacturer: Biovision

488.75 £

Antibody Target: Phospho-MSK1

Target Alternative Name: MSK1, MSPK1, MGC1911 , RLPK , RSKL

Tagline: 

Category: MSK1

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Clone: 

Species Reactivity: Human, mouse

Immunogen Sequence: Synthetic peptide surrounding phospho-Thr581 of human MSK1

Accession: O75582

Gene ID: 9252

Appearance: Colorless liquid

Antibody Form: Liquid

Concentration: 0.5 mg/ml

Formulation: 100 ug (0.5 mg/ml) affinity purified rabbit anti-Phospho-MSK1(Thr581) polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Purification: Affinity purified

Application: Western blot

Positive Control: 

Application & Usage: Western blot (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Phospho-MSK1(Thr581) detects 90 kDa MSK1 only when phosphorylated at Thr581. The antibody does not cross-react with nonphosphorylated MSK1 or MSK1 phosphorylated at other sites. It does not cross-react with RSK1, RSK2 or RSK3.

Background Descriptions: MSK1, a Mitogen and Stress activated protein Kinase, is activated by ERK as well as p38 MAPK in response to growth factors and various cellular stress stimuli. MSK1 resembles RSK by having two kinase domains connected by a regulator linker region. Phosphorylation of RSK1 at Ser 364, Ser381 and Thr574 is critical for RSK1 activity. These sites are analogous to Ser360, Ser376 and Thr581 of MSK1 and maybe important for MSK1 activity as well.

Storage Temperature: -20 Celsius

Shipping: gel pack

Shelf Life: 12 months

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use. Lyophilized and liquid form antibodies can be shipped and stored for short terms at +4 degrees Celsius and at -20 for long term storage.

Elisa: Not Verified

Western Blot (WB): Verified

Immunoprecipitation (IP): Not Verified

Immunofluorescence (IF): Not Verified

Flow Cytometry: Not Verified

Immunohistochemistry (IHC): Not Verified

Dot blot: 

ChIP: 

For more information, please, refer to the full datasheet.