Phospho-MAPKAPK-2 Antibody

Product Quantity: 100 ug Manufacturer: Biovision

488.75 £

Antibody Target: Phoshpo-MAPKAPK-2

Target Alternative Name: MAPKAPK2 ,MK2 , MAPKAPK-2

Tagline: 

Category: MAPKAPK

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Clone: 

Species Reactivity: Human, mouse

Immunogen Sequence: Synthetic peptide corresponding to residues surrounding Thr334 of human MAPKAPK-2

Accession: P49137

Gene ID: 9261

Appearance: Colorless liquid

Antibody Form: Liquid

Concentration: 0.5 mg/ml

Formulation: 100 ug (0.5 mg/ml) antigen affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Purification: Affinity purified

Application: Western blot

Positive Control: 

Application & Usage: Western blotting (1 ug/ml). However, the optimal conditions should be determined individually. The antibody detects phosphorylated MAPKAPK-2 (Thr334) of human and mouse origins. Reactivity to other species has not been tested.

Background Descriptions: MAP kinase-activated protein kinase-2 (MAPKAPK-2), also known as p45 hsp27 kinase, is a 45-54 kDa serine-threonine protein kinase. MAPKAPK-2 is rapidly phosphorylated and activated in response to stress, cytokines, and chemotactic factors. One of the major substrates of MAPKAPK-2 is hsp27, which stimulates actin polymerization in order to facilitate recovery from destruction of cytoskeleton during cellular stresses. Two isoforms are produced due to alternative splicing of the same gene which differs in their C-terminals. This antibody recognizes both isoforms.

Storage Temperature: -20 Celsius

Shipping: gel pack

Shelf Life: 12 months

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use. Lyophilized and liquid form antibodies can be shipped and stored for short terms at +4 degrees Celsius and at -20 for long term storage.

Elisa: Not Verified

Western Blot (WB): Verified

Immunoprecipitation (IP): Not Verified

Immunofluorescence (IF): Not Verified

Flow Cytometry: Not Verified

Immunohistochemistry (IHC): Not Verified

Dot blot: 

ChIP: 

For more information, please, refer to the full datasheet.