Phospho-ATF-2 Antibody

Product Quantity: 100 ug Manufacturer: Biovision

488.75 £

Antibody Target: Phospho-ATF-2 (Thr71)

Target Alternative Name: ATF2, HB16, CREB2, CREBP1, CRE-BP1, CRE-BP1, TREB7, MGC111558, Cyclic AMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; Activating transcription factor 2; cAMP response element-binding protein CRE-BP1; HB16; Cyclic AMP-responsive element-binding protein 2; cAMP-responsive element-binding protein 2; CREB-2.

Tagline: 

Category: ATF-2

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Clone: 

Species Reactivity: Human, mouse, rat

Immunogen Sequence: Synthetic phospho-peptide corresponding to residues surrounding Thr71 of human ATF2

Accession: P15336

Gene ID: 215274241

Appearance: Colorless liquid

Antibody Form: Liquid

Concentration: 0.5 mg/ml

Formulation: 100 ug (0.5 mg/ml) purified rabbit anti-Phospho-ATF-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Purification: Affinity purified

Application: Western blot, Immunoprecipitation, Immunohistochemistry

Positive Control: 

Application & Usage: Western blotting (1-4 ug/ml), immunoprecipitation (10 ug/ml), and Immunohistochemistry (20 ug/ml). However, the optimal conditions should be determined individually. The antibody detects phosphorylated ATF-2. It recognizes both Thr69/Thr71 dually phosphoralated ATF-2 as well as ATF-2 singly phosphorylated ATF-2 in samples of human, mouse, and rat origins.

Background Descriptions: The transcription factor ATF-2 (also called CRE-BP1) is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr69 and Thr71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr69 and Thr71 to prolong the half-life of ATF-2. ATF-2 also functions as a histone acetyltransferase (HAT) by specifically acetylating histones H2B and H4 in vitro.

Storage Temperature: -20 Celsius

Shipping: gel pack

Shelf Life: 12 months

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use. Lyophilized and liquid form antibodies can be shipped and stored for short terms at +4 degrees Celsius and at -20 for long term storage.

Elisa: Not Verified

Western Blot (WB): Verified

Immunoprecipitation (IP): Not Verified

Immunofluorescence (IF): Not Verified

Flow Cytometry: Not Verified

Immunohistochemistry (IHC): Not Verified

Dot blot: 

ChIP: 

For more information, please, refer to the full datasheet.