Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
- Tested Applications: WB
- Applications: This recombinant protein can be used for WB. For research use only.
- Predicted Molecular Weight: 51.4 kDa
- Physical state: Lyophilized
- Buffer: Tris with Glycine, Arginine and NaCl, pH7.5
- Concentration: N/A
- NCBI official symbol: Cd274
- Accession #: NP_068693
- Protein GI: N/A
- NCBI gene ID#: 60533
- NCBI official full name: CD274 antigen
- NCBI organism: Mus musculus
- Peptide sequence: N/A
- SWISSPROT #: Q9EP73
- Background Reference 1: Dong H., et al., 1999, Nat. Med. 5:1365-1369.
- Background Reference 2: Freeman G.J., et al., 2000, J. Exp. Med. 192:1027-1034.
- Background Reference 3: Nishimura H., et al., 2001, Trends in Immunology 22:265.
- Background Reference 4: N/A
- Background Reference 5: N/A
- Source: HEK293 cells
- Species: Mouse
- By Source: Human Cells
- By Species: Mouse
- Fusion tag: C-Fc Tag
- Sequence: Phe 19 - Thr 238
- Biology activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse PD-L1 / B7-H1 Protein, Fc Tag (1ug/well) can bind Biotinylated Mouse PD-1 / PDCD1, Fc Tag, Avi Tag (Avitag™). The linear range is 0.15-2.5 ug/mL.
- Purity: >92% as determined by SDS-PAGE.
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