PDL1 Antibody [4F2]

PD-L1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC) (1). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC (2). PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells (3). This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. PD-L1 also functions as an immune checkpoint protein, and multiple anti-PD-L1 antibodies are currently in phase II and III clinical trials, with one antibody already approved for the treatment of cancer (4).

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

• PD-L1 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Research area: Immunology
• Host species: Mouse
• Species reactivity: Human
• Homology: N/A
• Immunogen: PD-L1 antibody was raised against the extracellular domain of human PD-L1.
• Tested Applications: E, WB, IHC-P, ICC, IF, Flow
• Applications: PD-L1 antibody can be used for detection of PD-L1 by Western blot at 0.25 - 0.5 μ
• g/mL. Antibody can also be used for immunohistochemistry starting at 2 - 5 μ
• g/mL. For immunofluorescence start at 5 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunohistochemistry in human samples
• Immunocytochemistry in human samples
• Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1340 - Human Stomach Carcinoma Tissue Lysate
• Positive control 2: Cat. No. 1315 - Human Tonsil Tissue
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 32 kDa
• Observed: 45 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: PD-L1 Antibody is supplied as protein A purified IgG1.
• Clonality: Monoclonal
• Clone: 4F2
• Isotype: IgG1
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• NCBI official symbol: CD274
• Accession #: NP_054862
• Protein GI #: 7661534
• NCBI gene ID#: 29126
• NCBI official full name: programmed cell death 1 ligand
• NCBI organism: Homo sapiens
• SWISSPROT #: Q9NZQ7

Optimal dilutions for each application to be determined by the researcher.