p53 (Phospho-Ser6) Antibody
Short Name: Anti-p53 (Phospho-Ser6)
Long Name: p53 (Phospho-Ser6) Primary Polyclonal Antibody
Alternative Name: p53 (Phospho-Ser6) Polyclonal Antibody
Synonym Names: P53; BCC7; LFS1; TRP53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53
Also Known As: p53 (Phospho-Ser6) PAb
Conjugation: Unconjugated
Host: Rabbit (Oryctolagus cuniculus)
Target Antigen: p53 (Phospho-Ser6)
Specificity: This is a highly specific antibody against p53 (Phospho-Ser6).
Modifications: Phosphorylation
Modification Sites: Ser6
Clone number: Polyclonal antibody
Isotype: Immunoglobulin G (IgG)
Concentration:
Immunogen range:
Subcellular location:
Source: Synthetic phospho-peptide
Gene ID number: 7157
Swiss Prot: P04637
Tested Applications: WB
Recommended dilutions: WB (1:1,000)
Cross reactivity: Human
Cross reactive species details: Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the target antigen: Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
Purification method:
Storage conditions: Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20шC for up to 1 year.
