p53 Antibody (Clone C6A5)

Product Quantity: 100 ug Manufacturer: Biovision

448.25 £

Antibody Target: p53

Target Alternative Name: TP53, TRP53, P53, LFS1, Tumor suppressor p53, Phosphoprotein p53

Tagline: 

Category: p53

Host: Mouse

Antibody Type: Monoclonal

Isotype: Mouse IgG1

Clone: C6A5

Species Reactivity: Human, mouse, rat

Immunogen Sequence: Synthetic peptide corresponding to residue surrounding Ser20 of human p53

Accession: EAW90140

Gene ID: 7157

Appearance: Colorless liquid

Antibody Form: Liquid

Concentration: 0.5 mg/ml

Formulation: 100 ug (0.5 mg/ml) in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol.

Purification: Affinity purified

Application: Western blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry

Positive Control: 

Application & Usage: Western blotting (0.5-4 ug/ml), immunoprecipitation (4-8 ug/ml), Immunohistochemistry (4-8 ug/ml), immunocytochemistry (4-8 ug/ml). However, the optimal conditions should be determined individually.

Background Descriptions: p53, a 53 kDa protein, binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression through the cell cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells, principally due to the increased half-life (4 hrs) compared to that of wild-type (20 min). p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression, but may not always be necessary for apoptosis.

Storage Temperature: -20 Celsius

Shipping: gel pack

Shelf Life: 12 months

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use. Lyophilized and liquid form antibodies can be shipped and stored for short terms at +4 degrees Celsius and at -20 for long term storage.

Elisa: Not Verified

Western Blot (WB): Verified

Immunoprecipitation (IP): Not Verified

Immunofluorescence (IF): Not Verified

Flow Cytometry: Not Verified

Immunohistochemistry (IHC): Not Verified

Dot blot: 

ChIP: 

For more information, please, refer to the full datasheet.