NOD4 Antibody: NOD4 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD4 contains a CARD-like domain, a central NOD domain and a large LRR region. NOD4, an IFN-gamma-inducible nuclear protein, plays a role in homeostatic control of innate immunity and in antiviral defense mechanisms. As a key negative regulator of NF-κB and type I interferon signaling, NOD4 may be a useful target for manipulating immune responses against infectious or inflammation-associated diseases, including cancer.
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
- NOD4 antibody can be stored at 4˚
- C for three months and -20˚
- C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Research area: Innate Immunity
- Host species: Rabbit
- Species reactivity: Human, Mouse
- Homology: N/A
- Immunogen: NOD4 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human NOD4.
- The immunogen is located within amino acids 20 - 70 of NOD4.
- Tested Applications: E, WB, ICC, IF
- Applications: NOD4 antibody can be used for detection of NOD4 by Western blot at 1 μ
- g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ
- g/mL. For immunofluorescence start at 20 μ
- g/mL.
- Antibody validated: Western Blot in mouse samples
- Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- Specificity: NOD4 antibody is predicted to not cross-react with other members of the NOD family of proteins.
- Positive control 1: Cat. No. 1287 - EL4 Cell Lysate
- Positive control 2: Cat. No. 17-001 - HeLa Cell Slide
- Positive control 3: N/A
- Positive control 4: N/A
- Positive control 5: N/A
- Positive control 6: N/A
- Predicted molecular weight: 205 kDa
- MW explanation: N/A
- Validation: N/A
- Isoforms: N/A
- Purification: NOD4 Antibody is affinity chromatography purified via peptide column.
- Clonality: Polyclonal
- Clone: N/A
- Isotype: IgG
- Conjugate: Unconjugated
- Physical state: Liquid
- Buffer: NOD4 Antibody is supplied in PBS containing 0.02% sodium azide.
- Concentration: 1 mg/mL
- NCBI official symbol: NLRC5
- Accession #: NP_115582
- Protein GI #: 116268109
- NCBI gene ID#: 84166
- NCBI official full name: NLR family, CARD domain containing 5
- NCBI organism: Homo sapiens
- SWISSPROT #: Q86WI3
Optimal dilutions for each application to be determined by the researcher.
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