Nanos1 Antibody

Nanos1 Antibody: Nanos1 is one of three known mammalian homologs to the Drosophila gene nanos. Nanos1 is an RNA-binding protein containing a zinc-finger motif and is expressed in the developing nervous system and continues in the adult brain. Interestingly, unlike mice deficient in either nanos2 or nanos3, mice lacking the nanos1 gene develop normally with no sign of abnormalities. Recently it has been found that expression of nanos1 mRNA is down-regulated by E-cadherin in a human breast cancer cell line and the amino-terminal domain on Nanos1 interacts with the E-cadherin-binding protein p120ctn. Furthermore, overexpression of Nanos1 in human colorectal DLD1 cancer cells functionally abolished cell-cell adhesion, allowing the cancer cells to develop strong migratory and invasive properties. These results suggest that targeting Nanos1 might prove an effective strategy in the treatment of E-cadherin-negative tumors.

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

• Nanos1 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Research area: Signal Transduction
• Host species: Rabbit
• Species reactivity: Human
• Homology: Predicted species reactivity based on immunogen sequence: Mouse: (88%)
• Immunogen: Nanos1 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Nanos1.
• The immunogen is located within the first 50 amino acids of Nanos1.
• Tested Applications: E, WB, IHC-P, IF
• Applications: Nanos1 antibody can be used for detection of Nanos1 by Western blot at 1 μ
• g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1220 - SK-N-SH Cell Lysate
• Positive control 2: Cat. No. 10-301 - Human Brain Tissue Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: Nanos1 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: Nanos1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: NANOS1
• Accession #: Q8WY41
• Protein GI #: 41688589
• NCBI gene ID#: 340719
• NCBI official full name: nanos homolog 1 (Drosophila)
• NCBI organism: Homo sapiens
• SWISSPROT #: Q8WY41

Optimal dilutions for each application to be determined by the researcher.