NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Bacillus subtilis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Catalytic activity: ATP + NAD+ = ADP + NADP+
Stable for at least 6 months after receipt when stored at -80°C.
- Tested Applications: N/A
- Applications: This recombinant proteins is for research use only.
- Predicted Molecular Weight: N/A
- Physical state: Liquid
- Buffer: In 50mM TRIS-HCl, pH 7.0 containing 150mM sodium chloride, 1mM EDTA and 1mM dithiothreitol.
- Concentration: N/A
- NCBI official symbol: ppnK
- Accession #: O31612
- Protein GI: 8928487
- NCBI gene ID#: 936414
- NCBI official full name: inorganic polyphosphate/ATP-NAD kinase 1
- NCBI organism: Bacillus subtilis subsp. subtilis str. 168
- Peptide sequence: N/A
- SWISSPROT #: Q07011
- Background Reference 1: N/A
- Background Reference 2: N/A
- Background Reference 3: N/A
- Background Reference 4: N/A
- Background Reference 5: N/A
- Source: E. coli
- Species: N/A
- By Source: E. Coli
- By Species: Other
- Fusion tag: Tag Free
- Sequence: Full-length NAD kinase (Bacillus subtilis).
- Biology activity: ~2.6 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1umol nicotinamide adenine dinucleotide phosphate (NADP) per min.
- Purity: >98% (SDS-PAGE)
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