Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells (1). MYH3 (myosin, heavy chain, skeletal muscle, embryonic) plays a significant role in skeletal muscle development (2) and is also essential for the proper morphology and function of the developing heart (3). Mutations in this gene have been associated with Freeman-Sheldon syndrome and Sheldon-Hall syndrome (4).
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
- MYH3 antibody can be stored at 4˚
- C for three months and -20˚
- C, stable for up to one year.
- Research area: Homeostasis
- Host species: Rabbit
- Species reactivity: Human, Mouse, Rat
- Homology: N/A
- Immunogen: MYH3 antibody was raised against a 19 amino acid peptide near the amino terminus of human MYH3.
- The immunogen is located within amino acids 30 - 80 of MYH3.
- Tested Applications: E, WB, IHC-P
- Applications: MYH3 antibody can be used for detection of MYH3 by Western blot at 1 μ
- g/ml. Antibody can also be used for immunohistochemistry starting at 5 μ
- g/mL.
- Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
- Specificity: MYH3 antibody is human, mouse and rat reactive. MYH3 antibody is predicted to not cross-react with other members of the myosin heavy chain family.
- Positive control 1: Cat. No. XBL-10413 - Fetal Human Skeletal Muscle Lysate
- Positive control 2: N/A
- Positive control 3: N/A
- Positive control 4: N/A
- Positive control 5: N/A
- Positive control 6: N/A
- Predicted molecular weight: Predicted: 240 kDa
- Observed: 245 kDa
- MW explanation: N/A
- Validation: N/A
- Isoforms: N/A
- Purification: MYH3 antibody is affinity chromatography purified via peptide column.
- Clonality: Polyclonal
- Clone: N/A
- Isotype: IgG
- Conjugate: Unconjugated
- Physical state: Liquid
- Buffer: MYH3 antibody is supplied in PBS containing 0.02% sodium azide.
- Concentration: 1 mg/mL
- NCBI official symbol: MYH3
- Accession #: NP_002461
- Protein GI #: 98986453
- NCBI gene ID#: 4621
- NCBI official full name: myosin, heavy chain 3, skeletal muscle, embryonic
- NCBI organism: homo sapiens
- SWISSPROT #: P11055
Optimal dilutions for each application to be determined by the researcher.
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