LC3 (APG8) Antibody (Clone 166AT1234)

Product Quantity: 100 ul Manufacturer: Biovision

434.75 £

Antibody Target: LC3

Target Alternative Name: MAP1LC3A; Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; Microtubule-associated protein 1 light chain 3 alpha

Tagline: Monoclonal antibody to detect LC3 in human, mouse and rat samples

Category: LC3

Host: Mouse

Antibody Type: Monoclonal

Isotype: Mouse IgG1-Kappa

Clone: 166AT1234

Species Reactivity: Human, Mouse, Rat

Immunogen Sequence: This antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).

Accession: Q9H492

Gene ID: 84557

Appearance: Colorless liquid

Antibody Form: Liquid

Concentration: N/A

Formulation: Supplied in PBS with 0.09% (W/V) sodium azide.

Purification: Affinity Purified Antibody

Application: Western blot, Immunohistochemistry, IF

Positive Control: WB: Hela cell lysate, IHC: muscle tissue, IF: U251 cells

Application & Usage: Western blot: 1:1000, IHC: 1:50-100, IF: 1:200.

Background Descriptions: Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.

Storage Temperature: -20 Celsius

Shipping: gel pack

Shelf Life: 12 months

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use. Lyophilized and liquid form antibodies can be shipped and stored for short terms at +4 degrees Celsius and at -20 for long term storage.

Elisa: Not Verified

Western Blot (WB): Verified

Immunoprecipitation (IP): Not Verified

Immunofluorescence (IF): Verified

Flow Cytometry: Not Verified

Immunohistochemistry (IHC): Verified

Dot blot: Not Verified

ChIP: Not Verified

For more information, please, refer to the full datasheet.