IP3(Inositol Triphosphate) ELISA Kit

Supplier: Gentaur

651.95  GBP
Inc. VAT:782.34 GBP
Catalog: 855-ELK8148-48T
Product Size: 48T

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This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IP3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

  • Signal transduction
  • Metabolic pathway
  • Endocrinology
  • Cardiovascular biology
  • Neuro science
  • Hormone metabolism
  • Bone metabolism

Storage, Shipped at 4 °C


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