eNOS Antibody

Product Quantity: 100 ug Manufacturer: Biovision

380.75 £

Antibody Target: eNOS

Target Alternative Name: NOS3 , cNOS , ECNOS , EC-NOS , NOSIII

Tagline: 

Category: eNOS

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Clone: 

Species Reactivity: Human, mouse, rat

Immunogen Sequence: Synthetic peptide surrounding amino acid 602 of rat eNOS

Accession: EDL99377

Gene ID: 24600

Appearance: Colorless liquid

Antibody Form: Liquid

Concentration: 0.5 mg/ml

Formulation: 100 ug (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Purification: Affinity purified

Application: Western blot

Positive Control: 

Application & Usage: Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes 140 kDa eNOS of human, mouse, and rat origins. Reactivity to other species has not been tested.

Background Descriptions: eNOS (Endothelial nitric-oxide synthase) is an important enzyme in the cardiovascular system. eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495. Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli. In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation.

Storage Temperature: -20 Celsius

Shipping: gel pack

Shelf Life: 12 months

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use. Lyophilized and liquid form antibodies can be shipped and stored for short terms at +4 degrees Celsius and at -20 for long term storage.

Elisa: Not Verified

Western Blot (WB): Verified

Immunoprecipitation (IP): Not Verified

Immunofluorescence (IF): Not Verified

Flow Cytometry: Not Verified

Immunohistochemistry (IHC): Not Verified

Dot blot: 

ChIP: 

For more information, please, refer to the full datasheet.