Quantitative sandwich ELISA for measuring Rat Sonic hedgehog protein (SHH) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
- Sonic hedgehog (Shh) is a member of a small family of secreted proteins that are essential for development in both vertebrates and invertebrates. In mammals, there are three related hedgehog genes (sonic
- desert
- Indian) which share approximately 60 % amino acid (aa) identity. Human Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N-terminal fragment (Shh-N), and a catalytic-processing, glycosylated, 34 kDa, 239 aa C-terminal fragment.The 20 kDa Shh-N fragment is the core of the active hedgehog molecule. It begins with a Cys and ends with a Gly, and both amino acids are sites of lipid modification.
All of the components are shipped on ice packs/blue ice. Upon receiving the kit keep it at +4 degrees Celsius for up to 1 year (refer to the label on the box for the exact expiration date).
- Reactivity: Rat
- Sample types: serum, whole blood, plasma and other biological fluids
- Detection range: Please inquire
- Sensitivity: Please inquire Quantitative Sandwich ELISA
- Method of detection: Colorimetric
This kit is intended for research use only. It should not be used for human or animal clinical diagnostics or treatment.
0 reviews for Abbkine