Quantitative sandwich ELISA for measuring Rat Cross linked C-telopeptide of type I collagen (CTX-I) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
The carboxyterminal telopeptide of type I collagen (ICTP) is an indicator of degradation of type I collagen. ICTP antigen consists of a trivalent collagen cross-link joining three polypeptide chains of which two are ?1 chains of one collagen molecule while the third is derived from either an ?1 or an ?2 chain of the helical region of another molecule (Fig. 1) (Risteli et al. 1993). The major determinant for antigeneity must contain two phenylalanine-rich regions, which is only possible if the cross-link is trivalent in nature (Sassi et al. 2000). In osteoclasts, catepsin K cleaves the trivalently cross-linked ICTP structure at two sites between the phenylalanine-rich region and the cross-link, thereby destroying any reactivity with ICTP antibodies (Sassi et al. 2000). Therefore degradation of type I collagen in bone is not measurable by the present ICTP-test. ICTP is cleared from circulation by the kidneys and has a molecular mass of about 12 000?20 000 (Risteli et al. 1993).
All of the components are shipped on ice packs/blue ice. Upon receiving the kit keep it at +4 degrees Celsius for up to 1 year (refer to the label on the box for the exact expiration date).
- Reactivity: Rat
- Sample types: serum, whole blood, plasma and other biological fluids
- Detection range: Please inquire
- Sensitivity: Please inquire Quantitative Sandwich ELISA
- Method of detection: Colorimetric
This kit is intended for research use only. It should not be used for human or animal clinical diagnostics or treatment.
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