This Human Ubiquitin D (UBD) ELISA Kit employs a two-site sandwich ELISA to quantitate UBD.
Ubiquitin D is a protein encoded by the UBD gene.Using selective cDNA hybridization of a YAC containing the HLA-F locus region on chromosome 6, followed by Southern and Northern blot analyses, Fan et al. (1996) isolated a cDNA, which they called 1F12, encoding a protein similar to diubiquitin. The 1.1-kb transcript was detected only in B-cell lines transformed by Epstein-Barr virus.
By subtractive hybridization of dendritic cell (DC) libraries and EST database searching, Bates et al. (1997) obtained a full-length cDNA encoding diubiquitin. The predicted 165-amino acid protein has 2 ubiquitin-like domains, each of which has 2 cysteines. Southern blot analysis indicated that the cDNA represents a single-copy gene.
Storage: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.Shipping:Gel pack with blue ice.
- Reactivity: Human
- Sample types: serum, whole blood, plasma and other biological fluids
- Detection range: Please inquire
- Sensitivity: Please inquire Quantitative Sandwich ELISA
- Method of detection: Colorimetric
This kit is intended for research use only. It should not be used for human or animal clinical diagnostics or treatment.
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