Quantitative sandwich ELISA for measuring Human Semaphorin-3C (SEMA3C) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
The 616-amino acid human semaphorin E contains an N-terminal signal sequence of 15 amino acids that is followed by a semaphorin domain. In vitro translation of the semaphorin E cDNA yielded a 70-kD protein. The authors showed that semaphorin E is secreted by mammalian cells. By Northern blot analysis, semaphorin E was expressed as a 5.2-kb transcript in all human tissues tested.
CDDP-sensitive cells transfected with semaphorin E cDNA acquired CDDP-resistance. Semaphorin E was overexpressed in CDDP-resistant cell lines and was induced by diverse chemotherapeutic drugs and by x-ray and UV irradiation. Aberrant semaphorin E protein expression was detected immunohistochemically
All of the components are shipped on ice packs/blue ice. Upon receiving the kit keep it at +4 degrees Celsius for up to 1 year (refer to the label on the box for the exact expiration date).
- Reactivity: Human
- Sample types: serum, whole blood, plasma and other biological fluids
- Detection range: Please inquire
- Sensitivity: Please inquire Quantitative Sandwich ELISA
- Method of detection: Colorimetric
This kit is intended for research use only. It should not be used for human or animal clinical diagnostics or treatment.
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