A sandwich ELISA kit for detection of B-Lymphocyte Activation Antigen B7-2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Lymphocyte Activation Antigen B7-2 (LAB7-2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Lymphocyte Activation Antigen B7-2 (LAB7-2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Lymphocyte Activation Antigen B7-2 (LAB7-2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
- 10nm. The concentration of B-Lymphocyte Activation Antigen B7-2 (LAB7-2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
The Human LAB7-2 ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
- Species reactivity: Human
- Sensitivity: 0.274ng/mL
- Detection range: 0.156-10ng/mL
- Standard solution concentration: 10ng/mL
- ELISA type: Sandwich
- Assay length: 3h
This kit is intended for research use only. Do not use for diagnostics purposes.
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