A competitive Inhibition ELISA kit for detection of C-Type Natriuretic Peptide from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
A monoclonal antibody specific to C-Type Natriuretic Peptide (CNP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled C-Type Natriuretic Peptide (CNP) and unlabeled C-Type Natriuretic Peptide (CNP) (Standards or samples) with the pre-coated antibody specific to C-Type Natriuretic Peptide (CNP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of C-Type Natriuretic Peptide (CNP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of C-Type Natriuretic Peptide (CNP) in the sample.
The Human CNP ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
- Species reactivity: Human
- Sensitivity: 25.4 pg/mL
- Detection range: 62.5-4000 pg/mL
- Standard solution concentration: 4000 pg/mL
- ELISA type: Competitive Inhibition
- Assay length: 2h
This kit is intended for research use only. Do not use for diagnostics purposes.
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