Competitive ELISA Kit for 8-OHdG detection in Urine, Cell Lysates, Plasma, Sample matrices samples from all species
8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS (10-11).
The kit is transported on ice packs (+4ºC). Upon receipt store as per the instructions keep the components separately at +4ºC and -20ºC. Non-hazardous item according to both ADR and UN regulations.
- Assay type: Competitive ELISA (Enzyme-linked Immunosorbent Assay)
- Species reactivity: Species Independent
- Sample types: Urine, Cell Lysates, Plasma, Sample matrices
- Sensitivity: 0.59 ng/mL
- Assay range: 0.94 - 60 ng/mL
- Incubation t 1 hour
- Number of samples per kit: 39 samples in duplicate
- Method of detection: Colorimetric Assay
For research use only. Not to be used for human or animal treatment or diagnostics.